Wednesday, 30 August 2023

Microtome


 Microtome is specialized cutting instrument which accurately and repeatedly slices section from a block of embedded tissue.different kinds of microtomes are used to section paraffin and plastic embedded tissues.microtome also know as chondriosome or sacrosome.

Main parts of microtomes.


There are different microtomes.

  • Base(microtome body)
  • Knife attachment and blade
  • Material or tissue holder 
Types of microtomes

Rocking microtome
The rocking microtome is an extremely reliable instrument comprising of three moving parts.the lead screw mechanism incorporates quality precision engineering and the cutting stroke is controlled by a single control lever which minimises the operator skill required.

Rocking microtome allows you to cut section of plant and animal in block or batches.its simple,easy, and affordable, however it's light weight structure makes it difficult to prepare thin section.

Rotary rocking microtome
The rotary microtome made by is a manually operated cutting device for all types of work with paraffin and hard cutting technology in a field of biology, medicine and industry.it is used for making microscopic preparation for subsequent illumination.example human tissue.

Sledge microtome




A sledge microtome is a device where the sample is placed in to a fixed holder ( shuttle) which mean then moves backwards and forwards across a knife.modern sled microtome have the sled place upon a liner bearing,a microtome to readily cut many coarse section.
Used primarily for large blocks, hard tissue or whole mounts.

Cryo microtome

Cryo microtome is a sectioning instrument that allows for the cutting of extremely thin section upto 20 micron of frozen tissue or scaffold for microscopy and other applications.its used for cut histological slides.


Ultra microtome


The ultra microtome is designed for cutting by a mechanism similar to the paraffin microtome, but but it's much more sophisticated and precise.its mainly used for as a sample preparation method for transmission electron microscopy.

Vibrating microtome

Vibrating microtome operates by cutting using a vibrating blade, allowing the resultant cut to be made with less pressure than would be required for a stationary blade.its used for cut thin slices of tissue for examination under a microscope.

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